and Wong et al. reported the ethyl acetate fraction of L. indica leaves along with the mollic acid arabinose isolated from it induced growth inhibitory result and apoptosis in Ca Ski human cervical cancer cells. The current review aimed to investigate the complete phen olic information, antioxidant effect and cytotoxic exercise of L. indica leaves. The antioxidant potency of L. indica leaves are investigated, using three distinctive established in vitro testing programs, this kind of as scavenging action on DPPH radicals, cutting down power assay and superoxide dismutase action assay. The complete phenolic material with the leaf extracts was also accessed by Folin Ciocalteaus strategy. To our expertise, there is no antioxidant review reported for L. indica leaves. Consequently, the antioxidant exercise of L.
indica leaves was evaluated as it hasn’t been determined previously. In see from the standard usage of L. indica in cancer connected conditions as well as investigation by Hsiung et al. and Wong et al. which indicated the possible use of L. indica while in the remedy of Ca Ski human cer vical cancer cells, it had been therefore required to selelck kinase inhibitor even further ex pand this region of investigation to other cancer cell lines. In accordance to Malaysian Cancer Statistics, colorectal cancer is among the main cancers in Malaysia and a total of 2,866 cancer cases have been diagnosed amid Malaysians in Peninsular Malaysia during the yr 2006. On this examine, we evaluated the cytotoxic activity of the extracts against 3 colon cancer cell lines with varying molecular qualities, HT 29, HCT 15 and HCT 116.
The resulting data will definitely deliver scientific support upon the common usage of L. indica. Techniques Chemical substances and reagents Gallic acid, BHA, ascorbic acid, DPPH, potassium ferricyanide, Folin Ciocalteus phenol reagent, MTT, RPMI 1640 medium and McCoys 5A medium have been obtained hop over to here from Sigma Aldrich Company. Trichloroacetic acid, ferric chloride, ethanol, hexane and ethyl acetate have been bought from Merck Business. Foetal bovine serum, penicillin, streptomycin and fungizone had been from PAA Lab. SOD kit was bought from Sigma Aldrich Company. Plant sample assortment and identification The fresh leaves of L. indica were collected from Serem ban, Negeri Sembilan, Malaysia in February 2011.
The plants have been recognized by Dr Yong Kien Thai of Institute of Biological Sciences, Faculty of Science, University of Malaya, Malaysia as well as a voucher specimen was deposited with the herbarium of the Institute of Biological Sciences, Faculty of Science, Uni versity of Malaya, Kuala Lumpur, Malaysia. Preparation of extracts The extracts had been prepared as previously described. Briefly, the leaves of L. indica have been washed, dried and ground to fine powder. The dried, ground leaves were extracted with ethanol at space temperature yielding a dark green crude ethanol extract.