78–3 62μM were observed [20] The IC50 value for our CD44-targete

78–3.62μM were observed [20]. The IC50 value for our CD44-targeted liposome is slightly higher (approximately 9-10μM), but we have examined activity against a highly aggressive human melanoma cell line. In addition, as www.selleckchem.com/products/nu7026.html discussed earlier, using HA as a targeting moiety suffers from reduced selectivity as (a) the cell surface receptor RHAMM binds to HA just as avidly as CD44 [28, 29] and (b) HA binding Inhibitors,research,lifescience,medical to CD44 is not sensitive to distinct glycosylation patterns of this receptor,

while α1(IV)1263–1277PA binding is [41]. Eliaz and Szoka Jr. reported an IC50 value for nontargeted PEG liposomes of >172.4μM, similar to what we observed for nontargeted PEG liposomes with M14#5 melanoma cells (117.6μM; Figure 5). Potential DOX delivery in vivo, however, is quite different than in vitro when one considers circulation times. Unlike DOX encapsulated within PEGylated liposomes, free DOX is rapidly cleared from circulation, and therefore exposure to tumor cells is limited. In fact, it has previously been reported that free DOX is cleared 450-times Inhibitors,research,lifescience,medical faster than DOX encapsulated within PEGylated liposomes

[90, 91]. Furthermore, extravasated PEGylated liposomes experience enhanced retention within the tumor site, which has been attributed to a lack of functional lymphatic drainage Inhibitors,research,lifescience,medical in tumors [51, 92]. In the B16F10 mouse melanoma model, DOX incorporated within nontargeted liposomes showed little effect in reducing tumor size, while targeted liposomes significantly reduced tumor size (Figure Inhibitors,research,lifescience,medical 8). The improved activity was due to the selective

uptake of targeted liposomes by CD44-expressing cells rather than DOX released from disintegrated liposomes, as the targeted liposomes were more effective than the nontargeted liposomes (Figure 8), while both liposome types were of similar stability (Figures ​(Figures22–4). The liposomal formulation utilized here has been noted previously as being highly stable compared with Inhibitors,research,lifescience,medical other liposomal compositions [63]. Several prior studies have examined the efficacy of DOX encapsulated, targeted liposomes on mouse tumor models [22, 24, 93]. Most relevant to the present study, Peer and Margalit compared DOX encapsulated HA liposomes, DOX encapsulated liposomes, and saline [22]. Mice were injected with C-26 colorectal tumor cells and treated at 4, 12, and 19 days with 10mg/kg DOX. At day 31, tumor sizes were ~100, ~400, and ~1250mm3 for the HA liposome, Fossariinae liposome, and saline treatments. Thus, CD44 targeting via HA appeared to be effective. The relative reduction in tumor size by the HA liposomes compared with saline (~12.5-fold) was greater than seen here (~2-fold; Figure 8), but the DOX dose in the prior study was twice that of our treatments (10mg/kg versus 5mg/kg) and the tumor type was different (colorectal versus melanoma). It should be noted that the B16F10 tumor is highly aggressive, with a doubling time of less than 24h.

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