This cell is then said to be clonogenic. Single cells were plated and cultured for 10 days with CF 1:200 (Figure 2). Colony formation was absent in HCT-116 and MSTO-211, while HFF and Met-5A colony yields were unaffected. This shows that CF selectively inhibits the ability of HCT-116 and MSTO-211to selleck chemical grow into a colony. Figure 2 HFF, Met5A,

HCT116 and MSTO colony formation capacity upon CF treatment. Five hundred viable cells, pretreated for 48 h with CF (1:200) and CNTRL, were allowed to grow in normal medium for 10-14 days and then stained by crystal violet solution. The image is representative of three independent experiments. CF induces apoptosis in HCT-116 and MSTO-211 cell lines In order to confirm whether CF-induced growth inhibition was due to apoptosis, CF-treated and untreated HCT-116 and MSTO-211 cells were analyzed by flow cytometry. The G1 peak was increased in CF-treated HCT-116 cells. The percentage of G1 peak in control and CF-treated HCT-116 cells for 24 and 48 hours was 32.8 ± 0.8, 39.0 ± 0.19 and 48.6 ± 1.5, respectively (Figure 3A). The sub-G1 peak, which is indicator of apoptosis, was raised following 24 and 48 hours of CF-treated MSTO-211 cells. The percentage of this sub-G1 peak in control and CF-treated MSTO-211 cells for 24 and 48 hours was IWP-2 in vivo 2.5 ± 0.03, 11.2 ± 1.0 and 17.8 ± 2.0, respectively (Figure 3B), thereby suggesting apoptotic cell death.

Caspase-3 is expressed in cells as an inactive precursor from which the subunits of the mature caspase-3 are proteolytically generated during apoptosis. In our experiments we used a mouse monoclonal antibody raised against the full length caspase-3, so the reduction of the expression of caspase-3 indicates apoptosis. Expression of caspase-3 and cleavage of poly (ADPribose) polymerase (PARP) (the substrate of caspase-3, an early index of apoptosis) were detected in western blot (Figure 3C,D) in CF-treated HCT-116 and MSTO-211cells. These results show that

CF induces apoptosis in HCT-116 and MSTO-211 cells. These results show that CF induces apoptosis in HCT-116 and MSTO-211 cells. Figure 3 Effects of CF on the HCT116 and MSTO SAR302503 cell-cycle progression and apoptosis. Cell cycle analysis after propidium iodide staining was performed by flow cytometry in HCT-116 and MSTO cells untreated Astemizole (CNTRL) or treated with CF (1:200) for 24 and 48 h (CF24 h and CF48 h). The percentages of HCT-116 and MSTO cells in the different phases of cell cycle was reported in graph (A) and (B), respectively. Data are expressed as mean ± SD of at least three independent experiments. Western blot of total lysates indicates that the CF activates caspase-3 and PARP cleavage in HCT-116 (C) and MSTO (D) cells upon CF treatment (1:200) for 24 and 48 h versus the untreated control (C). γ tubulin was examined as a loading control. The image represents three independent experiments.