1% to 38% ( Fig. 1D; Supplemental Table 1). However, when all females were considered, acy3 expression and egg quality were not correlated ( Supplemental Figs. 2G and 3C). Two microarray features (20 K probe ID numbers 38561 and 48795) identified

as importin subunit alpha-8 (synonym: karyopherin alpha 7, kpna7) were > 2-fold higher expressed in fertilized eggs from the best quality female (2) compared with fertilized eggs from both of the lowest quality females (12 and 13) ( Table 2). qPCR showed that kpna7 transcript was detectable in the eggs of all females involved in the fertilized and unfertilized egg studies ( Figs. 3D and 4D). For both fertilized and unfertilized eggs, female 10 had the lowest kpna7 transcript expression (RQ of 1.0 for both studies; Supplemental Table 11 and Supplemental buy PD0325901 Table 13). In fertilized eggs, the two females with the highest

kpna7 transcript expression were females 5 and 2 (RQ values of 64.1 and 27.8, respectively), while for unfertilized eggs females 9 and 2 (RQ values of 67.8 and 41.8, respectively) had the highest kpna7 transcript expression ( Supplemental Table 11 and Supplemental Table 13). It is interesting to note that females 2, 5, and 9 all had below average total mortality at 7 dpf (15.7%, 36.6%, and 28.5%, respectively, compared with an learn more average of 50.7%) ( Fig. 1C; Table 4). However, the association of high kpna7 expression and egg quality was not consistent. Some females with above Celecoxib average egg

quality (e.g. females 3, 11, and 16) had relatively low kpna7 transcript expression ( Figs. 1C, 3D, and 4D). Further, when all females were considered, there was no correlation between kpna7 transcript expression and egg quality in either fertilized or unfertilized eggs ( Supplemental Figs. 2H and 3D). The hacd1 transcript was detectable in the fertilized and unfertilized egg from all females involved in the qPCR studies ( Figs. 3E and 4E). In the fertilized egg qPCR study, females 6 and 7 had the lowest hacd1 transcript expression (RQ of 1.0), and female 6 also had the lowest hacd1 expression in the unfertilized egg qPCR study ( Supplemental Table 11 and Supplemental Table 13). In both the fertilized egg and the unfertilized egg qPCR studies, the highest hacd1 transcript expression was measured for females 2, 5, and 9 (RQ values of 8.6, 8.4, and 11.0, respectively, for fertilized eggs; and RQ values of 8.2, 7.5, and 4.3, respectively, for unfertilized eggs) ( Figs. 3E and 4E; Supplemental Table 11 and Supplemental Table 13), all of which had below average total mortality at 7 dpf ( Fig. 1C; Table 4). As seen with kpna7, however, the association of high hacd1 expression with higher egg quality was not consistent, with some females with above average egg quality (e.g. females 3, 11, and 16) having relatively low hacd1 transcript expression ( Figs. 1C, 3E, and 4E).