As such, further research would be useful to investigate whether CMR can provide an ergogenic benefit during a field test that replicates field-based team games. Furthermore, as previous research suggests an increased perception of exercise intensity may hinder performance during field-based team games [13], investigation of the influence of CMR on subjective experiences during multiple P505-15 ic50 sprint exercise is required. The primary aim of our current study was to examine the effect of CMR on multiple sprint performance during a field-based exercise protocol. Secondary and tertiary aims included assessments regarding CMR on subjective experiences during multiple sprint

exercise. Methods Participants Eight physically active males (Age; 22 ± 1 y; 75.0 ± 8.8 kg; estimated VO2max 52.0 ± 3.0 ml/kg/min) volunteered to take part in the study. Seven of the participants habitually participated in field-based multiple MG-132 in vivo sprint sport such as football (i.e., soccer) and rugby, while the other was a recreationally active runner. After participants were briefed about the nature of the study, they provided written informed consent. The exclusion criteria included usage of Elafibranor clinical trial creatine supplements in the 12 weeks prior to the study, due to its influence on multiple sprint performance [14]. The ethics committee for the Department of Health at the University

of Bath approved, which was according to the Declaration of Helsinki. We have presented a schematic representation of the experimental conditions is presented in Figure 1. Figure 1 Schematic representation of the time line of study procedures. Preliminary measures and test familiarization Five days prior to

the first experimental trial, participants reported to an indoor sprints track for preliminary measurements including the participant’s height and body mass. During this visit each participant completed a progressive multistage shuttle run Chlormezanone test, which estimated maximal oxygen uptake [15]. Following this, each participant completed one 15 min section of the Loughborough Intermittent Shuttle Test (LIST) and one repeated sprint ability (RSA) test in order to familiarize themselves with the experimental tests. At the completion of this visit, participants were familiarized with the psychological scales used in this study. Experimental trials During each experimental condition, participants completed two trials consisting of a CMR and placebo (PLA) supplement administered in a randomized, counterbalanced order. To maintain blinding to the investigators and participants, all treatments were pre-labelled and subsequently dispersed by a non-affiliated researcher not participating in this trial. Experimental trials were conducted 7-9 days apart and at the same time of day. In the 24 h preceding the first experimental trial, participants were asked to record their diet and then replicate it before the second trial.