DPPH radicals are widely used in the model system to investigate the scavenging activity of several natural phytocompounds. The result of DPPH scavenging activity in this study read me indicates that the plant was potentially active. Methanol extract shows % age inhibition of 57.82 as compared to aqueous extract which shows 41.97% age inhibition at the highest concentration of 1000��g/mL (Figure 1). The DPPH contains an odd electron, which is responsible for purple color, and absorbance wavelength of 517nm [22]. The methanol and aqueous extracts of P. aculeate L. were estimated using potassium ferric cyanide reduction method. In this assay, the yellow color formed in the reaction is significant indicator of antioxidant activity. From the two extract, methanol extract shows high absorbance of 0.

669, then comes the absorbance of aqueous extract that is 0.63 at the highest concentration (Figure 2). In the CUPRAC assay, Cu(II)-Nc which is the main oxidizing agent gets reduced to colored Cu(I)-Nc chelate which shows maximum absorbance at 450nm. Figure 3 shows the maximum absorbance of 0.241 and 0.331 of methanolic and aqueous extract at higher concentration, whereas standard, that is, gallic acid, shows absorbance of 0.718 at the same concentration. In this assay, a higher absorbance indicates higher antioxidant activity. Singh et al. [23] studied antioxidant properties using DPPH assay of leaves extracts, that is, methanol, chloroform, ethyl acetate, and aqueousness of P. aculeate L., and found that different phytochemicals, present in the leaves, are responsible for the high antioxidant potential.

Figure 1DPPH radical scavenging activity of methanol and aqueous extracts of P. aculeata L. leaves (values are average of triplicate experiment and are represented as mean �� SE).Figure 2Reducing power of extracts of P. aculeata L. leaves (values are average of triplicate experiment and are represented as mean �� SE).Figure 3Antioxidant activity of different extracts of P. aculeata L. leaves and standard (gallic acid) by using CUPRAC assay.The extracts were assessed for their radical scavenging potential using site-specific and nonsite-specific deoxyribose degradation assay. In nonsite specific degradation assay, methanol and aqueous extracts show the inhibition of 71.232 and 72.019% at the same concentration (Figure 4). In the site-specific assay, methanol extract showed 48.

268% inhibition, whereas aqueous extract shows inhibition of 29.921% at 200��g/mL (Figure 5). The standard (gallic acid) shows the inhibition of 69.68 and 85.005% in site- and nonsite-specific degradation assay at the 200��g/mL. These results Anacetrapib show the potent antioxidant nature of different extracts of P. aculeata L. The antioxidant compounds are responsible for the reduction of ferric (Fe3+) form to ferrous (Fe2+) form.