Methods: Their ability to induce the production of immunoglobulin E (IgE) specific was evaluated using an assay of homologous passive cutaneous anaphylaxis. Furthermore, an allergen recognized as the lupine (Lupinus angustifolius) was included
as a control. Results: The analysis by SDS-PAGE of pigeon pea protein showed four polypeptide bands from 75 to 50 KDa and approximately to 35 KDa. Sensitization tests with both protein isolates, from green beans and dry beans of pigeon pea did not induce the production of specific IgE in the 77.8% and 75% of cases, respectively. The pigeon pea protein stimulated the production of immunoglobulin G (IgG), determined by a specific ELISA. Conclusion: The pigeon pea
protein showed a reduced capability as an allergen in this in vivo model; its use can be an alternative to lupine and soybean flour. Nevertheless, other tests in AZD9291 purchase animal models via the gastrointestinal tract and use of adjuvants are necessary. Key Word(s): 1. allergies; 2. leguminous; 3. pigeon pea; 4. proteins; Presenting Author: Md. Ariful Haque Mollik Corresponding Author: Md. Ariful Haque Mollik Affiliations: Peoples Integrated Alliance Objective: Scientists are searching for new leads from the natural sources to combat different diseases. Until recently an insignificant part of the plants has been scientifically evaluated for their medicinal values. The investigations were undertaken to discover new drugs from the natural sources. Celsia coromandelina J.König ex Rottb. belongs to the plant family Scrophulariaceae Juss. and is widely distributed Y-27632 datasheet throughout the Bangladesh. It is locally known as Kukurmota. It is locally used for the remedy of killer diseases as well as debilitating diseases. The investigations examined the anti-inflammatory, antioxidant, and antibacterial effects of its all-parts. Methods: The 75% ethanol extract was tested selleck kinase inhibitor for anti-inflammatory effect using the
carrageenan-induced edema in Wistar rats. Free radical scavenging, total antioxidant, and total phenol content were assessed spectrophotometrically. The extract was tested for antibacterial activities using the agar well diffusion method and micro dilution assays. Results: The 75% ethanol extract gave a maximal inhibition of edema by 75.50% at 30 mg/kg. The total antioxidant capacities expressed in terms of ascorbic acid was 0.610 mg/g dry weight. The total phenol in terms of tannic acid was 7.50 mg/g dry weight. The extract also demonstrated free radical scavenging activities yielding half maximal inhibitory concentration (IC50) value of 1.175 mg/mL. The all-parts extract however, showed selective antibacterial activities, inhibiting growth of two microorganisms: Bacillus subtilis Ehrenberg, and Bacillus thuringiensis Berliner. The minimum inhibitory concentrations (MICs) were 500 and 1000 μg/mL respectively.