No growth was observed on acetate, formate, methanol, monomethylamine selleck chem inhibitor and yeast extract with N2-CO2 or H2 atmosphere in the presence or absence of sulfur [1]. Nitrate, tryptone and yeast extract were used as nitrogen sources [1]. Growth of strain BSAT was inhibited by chloramphenicol, penicillin G and rifampicin at 100 ��g/ml but not by streptomycin when added before incubation at the optimum temperature [1]. Figure 2 Scanning electron micrograph of D. thermolithotrophum BSAT Chemotaxonomy The total lipid content of strain BSAT is about 6% of the total dry weight and is characterized by the presence of aminophospholipids and a phospholipid at about 66%, Rf 0.7 and 30%, R f 0.5, respectively, as well as minor compounds [1].

Gas chromatographic analysis of fatty acid components of both compounds revealed the presence of saturated and monounsaturated acyl chains [1]. The phosphoinositol contains C16:0 (15%), C18:1 (41%) identified as methyl-oleate, and C18:0 (44%) identified as stearate. The phosphoamino-positive compounds contained C16:0 (14%), C18:1 (43%), C18:0 (31%) and C20:0 (12%), as well as minor compounds [1]. Genome sequencing and annotation Genome project history This organism was selected for sequencing on the basis of its phylogenetic position [31], and is part of the Genomic Encyclopedia of Bacteria and Archaea project [32]. The genome project is deposited in the Genomes On Line Database [13] and the complete genome sequence is deposited in GenBank. Sequencing, finishing and annotation were performed by the DOE Joint Genome Institute (JGI).

A summary of the project information is shown in Table 2. Table 2 Genome sequencing project information Growth conditions and DNA isolation D. thermolithotrophum strain BSAT, DSM 11699, was grown anaerobically in DSMZ medium 829 (Desulfurobacterium medium) [34] at 70��C. DNA was isolated from 0.5-1 g of cell paste using Qiagen Genomic 500 DNA Kit (Qiagen 10262) following the standard protocol as recommended by the manufacturer without modifications. DNA is available through the DNA Bank Network [35]. Genome sequencing and assembly The genome was sequenced using a combination of Illumina and 454 sequencing platforms. All general aspects of library construction and sequencing can be found at the JGI website [36]. Pyrosequencing reads were assembled using the Newbler assembler (Roche).

The initial Newbler assembly consisting of 96 contigs in one scaffold was converted into a phrap [37] assembly by making fake reads from the consensus, to collect the read pairs in the 454 paired end library. Illumina GAii sequencing data (45.0 Mb) was assembled with Velvet [38] and the consensus sequences were shredded into 1.5 kb overlapped fake reads and assembled together with the 454 Carfilzomib data. The 454 draft assembly was based on 192.1 Mb 454 draft data and all of the 454 paired end data. Newbler parameters are -consed -a 50 -l 350 -g -m -ml 20.