Non-O1/non-O139 V. cholerae strains are highly heterogeneous with considerable serological diversity and vary in virulence properties. The presence of virulence genes amongst some environmental strains is significant, and environmental strains constitute a reservoir of potential pathogenic strains to human diarrhoeal infections [18–21]. Some non-O1/non-O139 strains carry key virulence genes, such as cholera toxin (CT) and toxin co-regulated pili (TCP), which are usually carried by epidemic strains [22]. Some may also carry other virulence factors such as the repeat-like toxin (RtxA) – a cytotoxin

and the heat-stable enterotoxin {Selleck Anti-diabetic Compound Library|Selleck Antidiabetic Compound Library|Selleck Anti-diabetic Compound Library|Selleck Antidiabetic Compound Library|Selleckchem Anti-diabetic Compound Library|Selleckchem Antidiabetic Compound Library|Selleckchem Anti-diabetic Compound Library|Selleckchem Antidiabetic Compound Library|Anti-diabetic Compound Library|Antidiabetic Compound Library|Anti-diabetic Compound Library|Antidiabetic Compound Library|Anti-diabetic Compound Library|Antidiabetic Compound Library|Anti-diabetic Compound Library|Antidiabetic Compound Library|Anti-diabetic Compound Library|Antidiabetic Compound Library|Anti-diabetic Compound Library|Antidiabetic Compound Library|Anti-diabetic Compound Library|Antidiabetic Compound Library|Anti-diabetic Compound Library|Antidiabetic Compound Library|Anti-diabetic Compound Library|Antidiabetic Compound Library|buy Anti-diabetic Compound Library|Anti-diabetic Compound Library ic50|Anti-diabetic Compound Library price|Anti-diabetic Compound Library cost|Anti-diabetic Compound Library solubility dmso|Anti-diabetic Compound Library purchase|Anti-diabetic Compound Library manufacturer|Anti-diabetic Compound Library research buy|Anti-diabetic Compound Library order|Anti-diabetic Compound Library mouse|Anti-diabetic Compound Library chemical structure|Anti-diabetic Compound Library mw|Anti-diabetic Compound Library molecular weight|Anti-diabetic Compound Library datasheet|Anti-diabetic Compound Library supplier|Anti-diabetic Compound Library in vitro|Anti-diabetic Compound Library cell line|Anti-diabetic Compound Library concentration|Anti-diabetic Compound Library nmr|Anti-diabetic Compound Library in vivo|Anti-diabetic Compound Library clinical trial|Anti-diabetic Compound Library cell assay|Anti-diabetic Compound Library screening|Anti-diabetic Compound Library high throughput|buy Antidiabetic Compound Library|Antidiabetic Compound Library ic50|Antidiabetic Compound Library price|Antidiabetic Compound Library cost|Antidiabetic Compound Library solubility dmso|Antidiabetic Compound Library purchase|Antidiabetic Compound Library manufacturer|Antidiabetic Compound Library research buy|Antidiabetic Compound Library order|Antidiabetic Compound Library chemical structure|Antidiabetic Compound Library datasheet|Antidiabetic Compound Library supplier|Antidiabetic Compound Library in vitro|Antidiabetic Compound Library cell line|Antidiabetic Compound Library concentration|Antidiabetic Compound Library clinical trial|Antidiabetic Compound Library cell assay|Antidiabetic Compound Library screening|Antidiabetic Compound Library high throughput|Anti-diabetic Compound high throughput screening| (NAG-ST) [4, 18, 22–26]. A novel type III secretion system (T3SS) was found in some non-O1/non-O139 strains and appears to be an important virulence factor [27–29]. The T3SS translocates a number of T3SS effectors into the host cell which interfere with host cell signalling [27, 28]. Shin et al.[29] showed that T3SS is an essential virulence factor for the non-O1/non-O139 strain AM-19226. In this study, 40 non-O1/non-O139 V. cholerae

isolates BIX 1294 chemical structure from hospitalised diarrhoeal patients in Zhejiang Province, China were analysed using multilocus sequence typing (MLST) and pulsed-field gel electrophoresis (PFGE) to determine their overall genetic relatedness. The presence of key virulence genes including enterotoxins, TCP and T3SS was also analysed. Results and discussion Isolation of non-O1/non-O139 V. cholerae

isolates from diarrhoeal patients in Zhejiang, China A total of 40 non-O1/non-O139 V. cholerae isolates was retrieved from different cities in Zhejiang Province, China, over a period of six years from 2005 to 2011 (Figure 1, Table 1). Nine isolates were from sporadic cases from seven cities, while 22 isolates were obtained from three outbreaks many in three different cities: outbreak A in Ningbo in 2005, outbreak B in Lishui in 2006 and outbreak C in Quzhou in 2011. The three outbreaks were notified as food poisoning events and were investigated. Outbreak A involved 20 cases with symptoms ranging from CX-5461 cell line cholera-like diarrhoea to mild diarrhoea and was initially suspected to be a cholera outbreak. Non-O1/non-O139 V. cholerae was isolated from nine patients. The outbreak occurred in a factory canteen and the food source of the outbreak could not be identified. Outbreak B involved eight cases, all having cholera-like symptoms. Non-O1/non-O139 V. cholerae was isolated from all but one patient. The source of the outbreak was traced to cross contamination of a cold dish from raw cuttlefish. Outbreak C occurred in a family function involving 12 cases with non-O1/non-O139 V. cholerae isolated from nine cases. The source of the outbreak was shrimp. Figure 1 Geographical map of Zhejiang Province, China. Cities are demarcated with dark solid lines.