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In A short course in bacterial genetics: a laboratory manuals and handbook for Escherichia coli and related bacteria. New York: Cold Spring Harbor Laboratory Press; 1992. Competing interests The authors declare that they have no competing interests. Authors’ contributions MGP carried out determinations of Cu2+ tolerance, polyP level, membrane electrical potential, Pi efflux, and gene expression. LASB initiated experiments in Cu2+ tolerance and polyP level measurements. MGP and LRM performed statistical analysis. MGP, MRR, and VAR prepared the manuscript and participated in the analysis of data. PD184352 (CI-1040) All authors designed the study and revised the manuscript for intellectual content. All authors read and approved the final manuscript.”
“Background The α-proteobacterium Rhodobacter capsulatus is one of several known species of prokaryotes that produces a gene transfer agent [1], or GTA. GTAs are phage-like particles that contain small fragments of the producing cells’ genomes [2] that can then be transferred to other cells in a process similar to generalized transduction. Production of the R. capsulatus GTA, RcGTA, is regulated through multiple cellular signal transduction systems. These include the GtaRI quorum selleck chemicals llc sensing system [3, 4] and the phosphorelay proteins CtrA and CckA [5, 6] and ChpT [6].