Results Gross glandular lesions were seen in 36 of the 63 stomachs examined (57.1%). The majority of lesions were seen in the antrum region (91.7%). In six stomachs, lesions were additionally or exclusively seen in the cardia or corpus region. No lesions were found in the duodenum. The lesions were classified in three groups as: Polypous (2 stomachs with polypoid masses located in both the cardia and the antrum with sizes between 1 and 5 centimetres in diameter), ii: Hyperplastic rugae lesions
(13 stomachs) or iii: Hyperaemic, erosive or ulcerative lesions, which were seen in 21 stomachs. The hyperplastic rugae were all seen in the antrum and ranged from having intense hyperemia with exudate to rugae with normally appearing mucosal surface. Gross Sapanisertib thickening of the antrum rugae was caused ��-Nicotinamide cost primarily by hyperplasia of the gastric foveolae compared to the respective normal samples. The remaining lesions were all found to be small solitary S3I-201 lesions of no more than approximately 1 × 2 cm in size. Focal areas
of erosive gastritis was the most common findings of these type lesions and characterised as sloughing of the superficial cells of the luminal epithelium with a concurrent fibrinopurulent exudate, luminal cellular debris and a predominantly mononuclear cell infiltrate of the lamina propria. Deeper erosions found in 9 stomachs eroded both the region of the gastric pits and parts of the glands, which was observed with gastritis only of the immediate tissues. One true ulcer was found extending the full thickness of the lamina propria, exposing the lamina muscularis to the lumen. A maximum of
two lesions were found in each of these stomachs. Helicobacter and Urease activity test Using the genus Helicobacter specific probe no positive signals were found in any of the 79 tissue samples (36 paired samples and 7 controls). In agreement with these results of the FISH, none of the samples tested positive for urease activity either. Internal controls of all urease Alectinib tests were found positive as indication of a functional test kit. Bacteria in general In general, only few bacteria were observed related to the mucosal surface in both the injured as well as in the healthy stomach samples. Overall, four morphological different types of bacterial cells could be visualized with the Eubacteria probe: 1) small, short (0.2-0.5 μm) coccoid rods, 2) distinct rods (1 × 3 μm), 3) long chained rods (up to 60 μm) or 4) large (2-3 μm diameter) coccoid bacteria clearly dividing in pairs. Typically when present, bacteria were observed in clusters associated with feed particles or located close to the mucosal surface Evidence of bacterial gastritis was found in one stomach lesion grossly characterised as a solitary erosion, 1 × 2 cm in size, the centre being hyperaemic and surrounded by a proliferative epithelial rim (Fig. 1).