The aim of this study was to use nested-PCR as an accurate and fast method to trace MAP in bull semen. Semen samples
from 112 bulls were collected and DNA was extracted. Then, Rigosertib nested-PCR was performed by specific primers for IS900 gene of MAP. The PCR products with 230 bp length were estimated as a positive. The frequency of MAP in semen samples were 12.50%. The results were showed nested-PCR is a good procedure with high efficiency for detection of intracellular bacteria such as M. paratuberculosis in bull’s semen samples. Thus, despite this abundance more attention to this disease in bulls to identify MAP quickly is essential.”
“Biomass is deemed to be the main source of renewable energy in Serbia. Over the past GW 572016 couple of years, considerable efforts have been made to develop a technology
which would enable biomass bales of various sizes and shapes to be used for energy production. A hot water boiler with cigarette type of combustion was constructed and used in the experimental investigation of biomass combustion phenomena. During the experiments performed, numerous parameters were measured: flue gas temperature, water temperature at the boiler inlet and outlet, while O-2, CO2, CO, SO2, and NOx content in the flue gas was measured at the boiler outlet. Experiments were performed with biomass feed rate of 0.12 kg/s, mean boiler output of 1.56 MW and mean excess air coefficient of 2.1. During the steady state boiler operation, exhaust gas temperature was measured to be around 150-160 degrees C and obtained CO and NOx emission rates were found to be quite acceptable. In addition, combustion of biomass bales in cigar burners was modelled by the means of appropriate numerical simulation. A good agreement between experimental and numerical results was obtained. (C) 2013 Elsevier Ltd. All rights reserved.”
“This study describes the concept of prevention and identifies the knowledge, perceived benefits and barriers, as well as the practices of early detection of breast cancer among women from different cultural backgrounds and socioeconomic levels.
A socioconstructivist qualitative selleck chemicals llc study was conducted in Barcelona. The study population consisted of women who were either native (Spanish) or immigrants from low income countries, aged 40 to 69 years. Narrations of the 68 informants were subjected to sociological discourse analysis. Place and culture of origin, social class and the migratory process can either facilitate or constitute barriers to breast cancer prevention. (C) 2012 Elsevier Ltd. All rights reserved.”
“This paper is motivated from the analysis of neuroscience data in a study of neural and muscular mechanisms of muscle fatigue. Multidimensional outcomes of different natures were obtained simultaneously from multiple modalities, including handgrip force, electromyography (EMG), and functional magnetic resonance imaging (fMRI).