The first processing step is a cleavage mediated by the Microprocessor

complex containing the Drosha nuclease and the RNA-binding protein DiGeorge critical region 8 (DGCR8). We previously reported that dimeric DGCR8 binds heme and that the heme-bound DGCR8 GSK461364 price is more active than the heme-free form. Here, we identified a conserved dimerization domain in DGCR8. Our crystal structure of this domain (residues 298-352) at 1.7 angstrom resolution demonstrates a previously unknown use of a WW motif as a platform for extensive dimerization interactions. The dimerization domain of DGCR8 is embedded in an independently folded heme-binding domain and directly contributes to association with heme. Heme-binding-deficient DGCR8 mutants have reduced pri-miRNA processing activity in vitro. Our study provides structural and biochemical bases for understanding how dimerization and

heme binding of DGCR8 may contribute to regulation of miRNA biogenesis.”
“Laparoscopic fundoplication for gastroesophageal reflux disease has been associated with excellent symptom control. Compared with medical treatment, laparoscopic Nissen fundoplication has shown favorable control of typical reflux symptoms. However, in approximately 2% to 17% of patients, surgical treatment fails. The role of reoperative repair for reflux disease and the factors that contribute buy PLX-4720 to it are examined. (J Thorac Cardiovasc Surg 2012;144:S71-3)”
“Study aim. – In this study, evoked potentials (EPs) to a pneumatic, innocuous, and calibrated stimulation of the skin were recorded in 22 volunteers.

Methods. – Air-puff stimuli were delivered through a home-made device (INSA de Lyon, Laboratoire Ampere, CHU de Saint-Etienne, France) synchronized with IWR-1 order an EEG recording (Micromecr).

Results. – A reproducible EP was recorded in 18 out of 22 subjects

(82% of cases) with a mean latency of about 120-130 ms, and maximal amplitude at Cz. This EP actually consisted of two components,, an auditory and a somatosensory one. Indeed, it was significantly decreased in amplitude, but did not disappear, when the noise generated by the air-puff was masked. We also verified that a stimulation close to the skin but not perceived by the subject wa’s not associated with any ER Conduction velocity between hand and shoulder was calculated around 25 m/s.

Conclusions. – This preliminary study demonstrates that pneumatic EPs can be recorded in normal volunteers. (C) 2013 Published by Elsevier Masson SAS.”
“A quantitative analysis of the direction of bending of two-stranded alpha-helical coiled coils in crystal structures has been carried out to help determine how the amino acid sequence of the coiled coil influences its shape and function.