tigurinus was detected by the specific RT-PCR, respectively. Overall, in 27 (53%) out of 51 individuals, S. tigurinus was detected in the saliva samples and/or in the plaque samples. In 13 (26%) individuals, S. tigurinus was detected both in the saliva and in the plaque samples. When comparing age groups <39 yr (n = 25), 40–65 yr (n = 16) and >65 yr (n = 10), no significant difference was observed for detection of S. tigurinus in the oral samples (P = 0.756). this website Systemic comorbidities of patients were as follows: diabetes mellitus (n = 5),

coronary heart disease (n = 3), rheumatoid arthritis (n = 1) and juvenile polyarthritis (n = 1); no immunosuppression was observed. Influence of periodontitis in the occurrence of S. tigurinus Clinical diagnosis of periodontitis was based on the PSI. Individuals of the non-periodontitis control group (n = 26) had PSI grades

<3 whereas patients of the periodontitis group (n = 25) had PSI grades 3 (n = 2) and 4 (n = 23). There is no significant difference of the frequency of S. tigurinus detection by RT-PCR in the saliva and dental plaque samples in the two groups: in the control group, 14 (54%) out of 26 individuals had S. tigurinus either GM6001 purchase in the saliva samples and/or in the plaque samples, and in the periodontitis group, 13 (52%) out of 25 patients had S. tigurinus in the mouth samples, respectively (P = 0.895) (Tables 1 and 2). Four (15%) out of 26 individuals of the non-periodontitis group and 9 (36%) out of 25 patients Adenosine triphosphate of the periodontitis group had S. tigurinus in both the saliva and the plaque samples, respectively (P = 0.091). Table 1 Frequency of S . tigurinus detected in

the oral microbial flora of the periodontally healthy subjects (n = 26) by specific RT TaqMan PCR Individual Age, sex Nicotine consumption Detection of S . tigurinus in saliva sample by RT-PCR Detection of S . tigurinus in subgingival plaque sample by RT-PCR 1 23, f Yes Negative Positive 2 23, f Yes Negative Negative 3 18, f No Negative Negative 4 18, f No Positive Negative 5 22, f Yes Positive Positive 6 16, f No Positive Negative 7 23, f No Positive Negative 8 18, f Yes Negative Negative 9 39, f Yes Positive Positive 10 16, f Yes Negative Negative 11 26, f No Negative Negative 12 26, m No Negative Negative 13 24, f No Negative Negative 14 48, m No Positive Negative 15 31, m Yes Negative Negative 16 53, m No Negative Negative 17 24, f No Positive Positive 18 26, f No Positive Negative 19 33, m No Negative Positive 20 58, m No Negative Negative 21 25, m No Positive Positive 22 23, m Yes Positive Negative 23 34, f No Negative Negative 24 25, f No Negative Negative 25 24, f No Negative Positive 26 25, f No Positive Negative Table 2 Frequency of S . tigurinus detected in the oral microbial flora of the periodontitis group (n = 25) by specific RT TaqMan PCR Patient Age, sex Nicotine consumption Detection of S . tigurinus in saliva sample by RT-PCR Detection of S .