We excluded patients who had myocardial infarction,

acute inflammatory disease, heart failure, cardiomyopathy, or pericardial effusion. Moreover, we excluded those whose transthoracic echocardiographic view was inadequate for measuring the epicardial fat thickness. On admission, blood sampling was performed to measure total cholesterol, Inhibitors,research,lifescience,medical triglyceride, high density lipoprotein (HDL)-cholesterol, low density lipoprotein (LDL)-cholesterol, fibrinogen and high-sensitivity C-reactive protein (hsCRP) in an overnight fasting state. Height (m2) and body weight (kg) were used to calculated body mass index (BMI). Coronary angiography In a fasting state, coronary angiography was performed by the Judkins’ method following the Perifosine puncture of femoral artery or via a radial artery approach. The severity of coronary atherosclerotic lesions was evaluated from at least Inhibitors,research,lifescience,medical three projections in all the patients. Significant stenosis was defined as a diameter stenosis of 50% or greater in major three epicardial arteries. Blood Inhibitors,research,lifescience,medical sampling for measuring the adiponectin level was obtained at the time of angiography.

Serum adiponectin was measured by ELISA (R&D Systems, Inc., USA). Measurement of echocardiographic cardiac adipose tissue Each patient underwent transthoracic echocardiography on the next day after coronary angiography. Echocardiograms were performed with VIVID 7 (GE, USA) instrument by standard techniques with subjects Inhibitors,research,lifescience,medical in the left lateral decubitus position. We measured EAT thickness on the free wall of right ventricle from

parasternal long-axis views. EAT was identified as an echo-free space in the pericardial layers on the two-dimensional echocardiography and its thickness was measured perpendicularly on the free wall of the right ventricle at end-diastole on three cardiac cycles.13),14) The measurement was performed at the Inhibitors,research,lifescience,medical point on the free wall of the right ventricle along the midline of the ultrasound beam, with the best effort to be perpendicular to the aortic annulus, anatomical landmark (Fig. 1). MAT presenting as an echo-lucent area above the parietal pericardium was also measured at same line. In thin patients whose MAT was indistinguishable, the free wall of the right ventricle was magnified for observation, and if it was still indistinguishable Sodium butyrate after magnification (11 cases), only PAT thickness was used in statistical analysis. The average value of three cardiac cycles from each echocardiographic view was used for the statistical analysis. The intra-observer and inter-observer correlation coefficients were 0.94 and 0.90, respectively, indicating good reproducibility and reliability. Fig. 1 Echocardiographic measurement of epicardial adipose tissue and mediastinal adipose tissue. EAT: epicardial adipose tissue, MAT: mediastinal adipose tissue. Statistics Statistical analysis was done using SPSS 11.0 for Windows.