Standard molecular mass markers are indicated. Distinct protein spots (n = 39) with specific IgG learn more immunoreactivity, as seen in corresponding immunoblots

(B), were subjected to tryptic digestion followed by MALDI-TOF-MS analysis for identification (marked with arrow). The 17 proteins identified are numbered and listed in Table 2. Spot No. 2A-2 M was identified as thioredoxin reductase GliT. Table 2 Immunoreactive proteins of A.fumigates identified by MALDI-TOF-MS Spot no. Accession No. (GenBank) Organism Protein name Peptides matched Sequence coverage(%) Mascot score BLAST score (E-value) Theoretical pI/Mr(kDa) Probable functions 1A-1H GI:71001112 Aspergillus fumigatus Af293 secreted dipeptidyl peptidase DppV 26 33 135 1.60E-08 5.59/79.7 Metabolism of dipeptides 2A-2M GI:70992029 Aspergillus fumigatus Af293 thioredoxin reductase GliT 20 Saracatinib chemical structure 54 149 6.30E-10 5.44/36.2 Provide self-protection to A. fumigatus 3 GI:159123228 Aspergillus fumigatus A1163 FAD dependent oxidoreductase, putative 25 44 173 2.50E-12 5.94/51.5 Oxidoreductase 4 GI:70989411 Aspergillus fumigatus Af293 fumarylacetoacetate

PF299 mw hydrolase FahA 13 37 85 0.0015 5.95/46.9 Phenylalanine catabolism, Tyrosine catabolism 5 GI: 119492487 Neosartorya fischeri NRRL 181 aspartyl aminopeptidase 20 40 98 8.90E-05 6.03/53.9 proteolysis, tissue invasion 6A-6B GI: 70992355 Aspergillus fumigatus Af293 aldehyde dehydrogenase AldA 25 54 171 4.00E-12 6.30/61.4 Alcohol metabolism 7 GI: 71002030 Aspergillus fumigatus Af293 aromatic aminotransferase Aro8 19 52 145 3.10E-08 5.96/58.3 Aromatic aminoacid family metabolic process 8A-8B GI: 70999466 Aspergillus fumigatus Af293 fructose-bisphosphate

aldolase, class II 19 62 137 9.90E-09 5.55/39.9 Glycolysis, Carbohydrate degradation 9 GI: 119499942 Neosartorya fischeri NRRL 181 G-protein comlpex beta subunit CpcB 18 59 130 5.00E-08 6.06/35.3 Receptor signaling, intracellular signal transduction pathways, and protein synthesis 10 GI: 71001310 Aspergillus fumigatus Af293 actin cytoskeleton protein (VIP1) 13 40 86 0.0013 5.93/28.3 Component of cytoskeleton 11 GI: 159129975 Aspergillus fumigatus A1163 phytanoyl-CoA dioxygenase family 15 64 109 6.30E-06 6.08/33.7 Oxidization 12 GI: 70988713 Aspergillus fumigatus Af293 second pectate lyase A 13 44 96 0.00014 6.23/33.8 Carbohydrate metabolism, cell wall biogenesis/degradation 13 GI: 71001408 Aspergillus fumigatus Af293 urate oxydase UaZ 12 32 80 0.0052 7.24/34.1 Metabolism of urate 14 GI: 70986899 Aspergillus fumigatus Af293 malate dehydrogenase, NAD-dependent 23 70 258 7.90E-21 9.08/35.8 Cellular carbohydrate metabolic process 15 GI: 169764553 Aspergillus oryzae RIB40 hypothetical protein 13 41 92 2.90E-04 6.21/35.3 unknown 16A-16B GI: 70982195 Aspergillus fumigatus Af293 3-hydroxybutyryl-CoA dehydrogenase 15 44 90 0.00052 6.33/36.